PCR Reagent





BR Biochem Brand


We BR Biochem are specialized in all kinds of Molecular Biology products. We have huge range of DNA Ladders , Protein Marker , PCR Reagents, Taq DNA Polymerase , Dntp’s , PCR Kit etc. We have many satisfied customer who are using our product from a very long time.









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DNA Ladders


Cat No: BM014-10
Description

The 10bp DNA Ladder is ideal for determining the size of double-stranded DNA from 80 to 300 base pairs. The ladder consists of 18 linear double-stranded fragments. The 100bp and 200bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 100bp and 200bp are 40ng. The 100bp and 200bp fragment are 100ng. This ladder is pre-mixed with loading dye and is ready to use.

Recommended Loading: 2-5 μl/Lane
Concentration Typical Bands: 100 ng/5μl
Concentration Other Bands: 40 ng/5μl
Advised Electrophoresis Condition: 8 cm,5% Agarose Gel, 0.5×TBE, 5 V/cm, 1 h
Contents bp: 80, 90, 100, 110, 120, 130,140, 150, 160, 170, 180, 190, 200, 210, 220,230, 240, 250, 260, 270, 280, 290,300
Mix Concentration: 168 ng/μl
Storage: -20°C

Cat No: BM014-20
Description

The 20bp DNA Ladder is ideal for determining the size of double-stranded DNA from 60 to 300 base pairs. The ladder consists of 13 linear double-stranded fragments. The 100bp and 200bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 100bp and 200bp are 40ng. The 100bp and 200bp fragment are 100ng. This ladder is pre-mixed with loading dye and is ready to use.

Recommended Loading: 2-5 μl/Lane
Concentration Typical Bands: 100 ng/5μl
Concentration Other Bands: 40 ng/5μl
Advised Electrophoresis Condition: 8 cm,5% Agarose Gel, 0.5×TBE, 5 V/cm, 1 h
Contents bp: 60, 80, 100, 120, 140, 160,180, 200, 220,240, 260, 280, 300
Mix Concentration: 128 ng/μl
Storage: -20°C

Cat No: BM001-R500
Description

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 12 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-3,000 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.54 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.

Range: 100-3,000 bp
Number of bands: 12
Concentration: 108 μg/ml
Package: 54ug/500ul
Recommended Load: 5 μl / well
Storage: RT and 4°C up to 6 month.
Store at -20°C up to 1 year. Containing orange G & xylene cyanol FF
as tracking dyes.

Cat No: BM010-R500
Description

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 12 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-3,000 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.54 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.

Range: 250-10,000 bp
Number of bands: 13
Concentration: 100 μg/ml
Package: 50ug/500ul
Recommended Load: 5 μl / well
Containing bromophenol blue & xylene cyanol FF as tracking dyes.

Cat No: BM012-R500
Description

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 17 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 50-1,500 base pairs. The 200, 500 and 1200 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.56 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.

Range: 50-1,500 bp
Number of bands: 17
Concentration: 112 μg/ml
Package: 56ug/500ul
Recommended Load: 5 μl / well
Storage: -20°C
Containing orange G as tracking dyes.

Cat No: BM013-R500
Description

A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 14 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 250-25K base pairs. The 1K and 3K bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.52 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.

Range: 250-25K bp
Number of bands: 14
Concentration: 104 μg/ml
Package: 52ug / 500ul
Recommended Load: 5 μl well
Storage: 25°C for 6 months,
Store at 4°C for 12months, Store at -20°C for 24 months

Cat No: BM110-100
Description

OneMARK B with the Novel Green was designed to show virtually uniform spacing over a wide fragment range. The ladder is supplied in a ready-to-use format containing the fluorescent DNA stain and tracking dyes. High quantum yield and excellent stability make the fluorescence dye the ideal fluorophore for DNA staining applications and a superior replacement for the widely used dyes, ethidium bromide or SYBR® Green I.
The OneMARK B with the Novel Green was optimized for direct loading onto unstained agarose gels.
The ladders provide the highest level of convenience during the routine handling and avoid commonly used gel staining procedures with the ethidium bromide or SYBR® Green I. The OneMARK B includes fragments ranging from 250-10,000 base pairs. The 1K and 3K bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg per loading) for approximating the mass of DNA in comparably intense samples of similar size.

Range: 250-10,000 bp
Number of bands: 13
Concentration: 83.3 μg/ml
Package: 50ug/600ul
Recommended Load: 6 μl / well
Containing bromophenol blue and xylene
cyanol FF as the tracking dyes.
Storage: 4°C up to 6 month,
Store at -20°C up to 1 year.

Cat No: BM101-100
Description

OneMARK 100 with the Novel Green was designed to show virtually uniform spacing over a wide fragment range. The ladder is supplied in a ready-to-use format containing the fluorescent DNA stain and tracking dyes. High quantum yield and excellent stability make the fluorescence dye the ideal fluorophore for DNA staining applications and a superior replacement for the widely used dyes, ethidium
bromide or SYBR® Green I.
The OneMARK 100 with the Novel Green was optimized for direct loading onto unstained agarose gels.
The ladders provide highest level of convenience during the routine handling and avoid commonly used gel staining procedures with ethidium bromide or SYBR® Green I. The OneMARK 100 includes fragments ranging from 100-3,000 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.54 μg per loading) for approximating the mass of DNA in comparably intense samples of similar size.

Range: 100-3,000 bp
Number of bands: 12
Concentration: 90 μg/ml
Package: 54ug/600ul
Recommended Load: 6 μl / well
Containing orange G, xylene cyanol FF as the tracking dyes.
Storage: 4°C up to 6 month, Store at -20°C up to 1 year.

Cat No: BM011-R500
Description

A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 19 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-10,000 base pairs. The 500, 1.5K and 3K bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.86 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.

Range: 100-10K bp
Number of bands: 19
Concentration: 100 μg/ml
Package: 86ug / 500ul
Recommended Load: 5 μl well
Containing orange G, xylene cyanol FF and bromophenol blue as tracking dyes.

Protein Marker


Cat No: BM005-0500
Description

The PiNKPlusPrestained Protein Ladder contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa. The PiNKPlusPrestained Protein Ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.
• Ready-to-use: supplied in a loading buffer for directloading on gels
• Easy to identify: includes the ~10, ~40 and ~90 kDareference bands coupled with an blue dye.
• Sharp bands.

Quality Control: Tested in SDS-polyacrylamide gelelectrophoresis and Western blotting.
Recommendations for Loading:
1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
2. Mix thoroughly to ensure the solution is homogeneous.
3. Load the following volumes of the ladder on SDS-polyacrylamide gel:
- 5 μl per well for mini-gels, 2.5 μl per well for blots
- 140 μl per well for large gels, 5 μl per well for blots.

Cat No: BM007-0500
Description

The BLUeyePrestained Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).The BLUeyePrestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.

• 3 μl or 5 μl per loading for clear visualization.
during electrophoresis on 15-well or 10-well minigel,respectively.
• 1.5~2.5 μl per well for general Westerntransferring.
Contents: Approximately 0.1~0.4 mg/ml of eachprotein in the buffer (20 mMTris-phosphate, pH.
7.5 at 25°C), 2 % SDS, 1 mMDithiothreitol, 3.6 MUrea, and 15 % (v/v) Glycerol).
Quality Control: Under suggested conditions, BLUeyePrestained Protein.
Ladder resolves 12 major bands in 15% SDS-PAGE (Trisglycine buffer) andafter Western blotting to nitrocellulose membrane.Guide for Molecular Weight Estimation (kDa).

Cat No: BM008-0500
Description

The BlueRAYPrestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Trisglycine buffer). The BlueRAYPrestained Protein Ladder is designed for monitoring protein separated during SDS polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.

• 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on 15-well or 10-well mini-gel, respectively.
• 1.5~2.5 μl per well for general Westerntransferring.
• Apply more for thicker (> 1.5 mm) or larger gel.
Contents: Approximately 0.1~0.4 mg/ml of eachprotein in the buffer (20 mMTris-phosphate, pH 7.5 at 25°C), 2 % SDS, 10 mMDithiothreitol, 3.6M Urea, and 15 % (v/v) Glycerol).
Quality Control: Under suggested conditions, BLUelfPrestained
Protein Ladder resolves 13 major bands in polyacrylamide gel withappropriate buffers and after Western blotting to nitrocellulosemembrane.

Cat No: BM003-0500
Description

The BLUltraPrestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa. Proteins are covalently coupled with a blue chromophore except for three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa) when separated on SDS-PAGE (Tris-glycine buffer). The BLUltraPrestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.

• Broad range: 6.5-270 kDa.
• Ready-to-use: supplied in a loading buffer fordirect loading on gels.
• Proteins are covalently coupled with a bluechromophore except for three referencebands (two orange bands at 30 kDa and 270kDa and one green band at 52 kDa).

Cat No: BM006-0500
Description

The BlueRAYPrestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Trisglycine buffer). The BlueRAYPrestained Protein Ladder is designed for monitoring protein separated during SDS polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.

• Broad range: 10-180 kDa.
• 3 μl or 5 μl per loading for clearvisualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
• 2~3 μl per well for general Westerntransfering.
• Apply more for thicker (>1.5 mm) orlarger gel.

Cat No: BM019-0500
Description

The BlueAQUAPrestained Protein Ladder is a blue protein standard with 11 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-glycine buffer). The BlueAQUAPrestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.

• Broad range: 10-180 kDa
• 3 μl or 5 μl per loading for clearvisualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
• 1.5~2.5 μl per well for general Westerntransferring.
• Apply more for thicker (> 1.5 mm) orlarger gel.

TAQ DNA POLYMERASE

Cat No: BCQ050
Description

Taq DNA polymerase is a thermostable enzyme isolated from E. coli which encodes Taq DNA polymerase gene. This enzyme contains 5’-3’ poly merase and 5’-3’ exonuclease activity .

Storage buffer
50mM Tris-HCl pH7.9, 50mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mM PMSF , 50% glycerol.
10X reaction buffer

Containing 15mM MgCl2
Unit description
One unit is defined as the amount of enzyme that will incorporate 10nmole of dNTP into acid-insoluble material in 30 minutes at 74ºC.

PCR result


Applications
• Amplification of DNA fragments up to 5 kb
• Labeling of PCR products with modified nucleotides (biotin-dUTP, fluorescein-dUTP)
• Cycle sequencing
• Generation of PCR product for TA cloning

Cat Number : TQ050
Description

Ultra-Pure Taq DNA Polymerase (10X PCR Buffer is premixed with MgCl2) is a thermostable enzyme which is purified to reduce levels of contaminating DNA, making it well suited for PCR and sensitive experiments using bacterial templates or random primers. The high purity of this Taq DNA Polymerase makes it ideal in detecting and identifying bacterial DNA, and is a more accurate method for mutation scanning techniques while preventing the amplification of undesired DNA sequences. Ultra-Pure Taq DNA Polymerase is suitable for work in bacterial genomics due to the reduced probability of contamination leading to non-specific amplification or artifacts during PCR reactions. The amplified products are up to 8 kb with 3’ adenosine residues and are ready to use directly in TA cloning.

Cat Number : TQ050
Description

Hi Fidelity polymerase is a mixture of thermostable enzymes. It is specifically developed to synthesize length of PCR product up to 25 kb and with low error rate. Hi Fi polymerase synthesizes higher yields of product from genomic DNA, cDNA, and bacterial cultures. It has 2.5 hours half life at 96oC and easily amplify PCR product of G-C rich or secondary structure DNA by adding G- C rich buffer.

10X reaction buffer
Buffer containing 25mM MgCl2

Unit description
One unit is defined as the amount of enzyme that will incorporate 10nmole of dNTP into acid-insoluble material in 30 minutes at 74oC.
PCR result
dna
Hi Fidelity polymerase amplified λ DNA from 2.5k to 30k DNA fragement.

Cat Number : BCH0500
Description

Hot start Taq DNA Polymerase is designed for Real-Time PCR and Hot- start PCR. It is modified with a special inhibition of PCR at room temperature. This will prevent primer dimers and other artifacts.

Storage buffer
Description

50mM Tris-HCl pH7.9, 50mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mM PMSF , 50% lycerol.

10X reaction buffer
Buffer containing 25mM MgCl2
Unit description

One unit is defined as the amount of enzyme that will incorporate 10n mole of dNTP into acid-insoluble material in 30 minutes at 74oC.

Applications
- Hot Start and real time PCR
- Multiplex PCR
- Amplification of complex genomic and cDNA
dna

Cat Number : BCB0093
Description

Pfu DNA polymerase is a thermostable enzyme isolated from Pyrococcus furiousus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5'→3' direction. Pfu DNA polymerase possesses 3'→5' exonuclease (proofreading) activity. Base misincorporation is rapidly excised by the proofreading activity of the polymerase. Pfu DNA polymerase is recommended for PCR and primer extension reactions that require high-fidelity. The fragments of Pfu DNA polymerase generated are blunt-ended.

Unit description
One unit of Pfu DNA Polymerase incorporates 10 nmol of dNTP into acid-insoluble material in 30 min at 74°C.

Storage buffer
50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at -20ºC,100 mM KCl, 0.1 mM EDTA.
PCR result

dna

Pfu DNA polymerase amplified 1k to 7.5k DNA fragement

dNTP'S


Cat Number : EN-1
Description

10 mMdNTPs Mix is a ready-to- use solution of dATP, dCTP, dGTP and dTTP (monosodium salts) at a concentration of10 mM each in sterile deionized water at pH7.5, whose purity is ≥99% (HPLC). It is free of RNase and DNase, and issuitable for any molecular biology application that requires pure deoxynucleotides, such as PCR, DNA sequencing, cDNAsynthesis and nick translation.

Storage: Store at -20℃.

Cat Number : EN-2
Description

dNTPs Set contains 4×0.4ml of dATP, dCTP, dGTP and dTTP (monosodium salts) at a concentration of 100mM each in sterile deionized water at pH7.5, whose purity is up to 99.5% (HPLC). It is free of RNase and DNase, and suitable for any molecular biology application that requires pure deoxynucleotides, such as PCR, DNA sequencing, cDNA synthesis and nick translation.

Storage: Store at -20℃.

LOADING DYE


Cat Number : BCLD005
Description

Gel Loading Dye (6X) is a pre-mixed Loading Buffer with two tracking dye for agarose and non-denaturing poylacrylamide gel electrophoresis. EDTA is included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. Bromophenol blue and xylene cyanol are the standard tracking dye for electrophoresis.

Contents
10 mM Tris-HCL(pH 7.6)
0.03% Bromophenol Blue
0.03% Xylene Cyanol
60% Glycerol
60 mM EDTA
Storage: Store at RT

Cat Number : BCLD051
Description

Gel Loading Dye, Blue (6X) is a pre-mixed Loading Buffer with one tracking dye for agarose and non-denaturing poylacrylamide gel electrophoresis. EDTA is included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. Bromophenol blue is the standard tracking dye for electrophoresis.

Contents :
Gel Loading Dye, Blue (6X):
60 mM EDTA
0.03% Bromophenol Blue
60% Glycerol
60% Glycerol
10 mM Tris-HCL(pH 7.6)
Storage: Store at RT

Cat Number : BCLD061
Description

Gel Loading Dye, Three-colour (6X) is a pre-mixed Loading Buffer with three tracking dye for agarose and non-denaturing poylacrylamide gel electrophoresis. EDTA is included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. Bromophenol blue, xylene cyanol and orange G are the standard tracking dye for electrophoresis.

Contents
Gel Loading Dye, Three-colour (6X):
0.03% Bromophenol Blue
0.03% Xylene Cyanol
0.15% Orange G
60% Glycerol
60 mM EDTA
Storage: Store at RT

Cat Number : BCLD071
Description

Gel Loading Dye, Orange (6X) is a pre-mixed Loading Buffer with two tracking dye for agarose and non-denaturing poylacrylamide gel electrophoresis. EDTA is included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. Xylene cyanol and Orange G are the standard tracking dye for electrophoresis.

Contents
Gel Loading Dye (6X):
10 mM Tris-HCL(pH 7.6)
0.15% Orange G
0.03% Xylene Cyanol
60% Glycerol
60 mM EDTA
Storage: Store at RT

All-In-One PCR Master Mix


Cat Number : BCMB203-0100
Description

OnePCRTM is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. OnePCRTM is a pre-mixed solution containing Taq DNA polymerase, PCR Buffer, dNTP, gel loading dyes, and fluorescence dye. OnePCRTM which contains the Taq DNA polymerase, is purified from the E. coli., and expressing the Thermus aquaticus DNA polymerase gene. This enzyme has a 5' → 3' DNA polymerase and the 5' → 3' exonuclease activity but lacks the 3' → 5' exonuclease activity. OnePCRTM, which contains the fluorescence dye, is directly detected on BLooK LED transilluminator or UV epi-illuminator after the DNA electrophoresis. OnePCRTM mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents are provided with the sufficient amplification reactions of 50 μl each.

pcr

Features
No post-staining processing of DNA required.
No need to prepare PCR Reagents.
Direct loading onto your agarose gel for analysis.
Sensitivity – High degree of sensitivity as the ethium bromide.
Speed – No destaining requirement.
Compatibility – Use the blue light or UV to detect the signal.

Cat Number : BCMB200-P0100
Description

2X PCR SuperMix is a cocktail of calculated amounts of Mg++, dNTPs, and recombinant Taq DNA Polymerase for highly efficient amplification of nucleic acid templates by the polymerase chain reaction (PCR). 2X PCR SuperMix is supplied at a 2X concentration that allows the easy calculation of its final concentration for adding the primer and template to the mixture. The supplied reagent is sufficient for 100 amplification reactions of 50 μL each. The 2X PCR SuperMix is very stable, and no detectable reduction of PCR performance or enzyme activity is observed after storage for 12 months at 4°C. Repeated freeze-thaw cycles do not reduce performance or activity. Prolonged 37°C storage: enzyme activity maintains the same level after 21 days at 37°C.

pcr

● Super high speed - One-minute extension
➤ Template: Lambda
➤ DNA Primer: FW #1469; RV1kb #1472, 2kb #1473, 3kb #1474

pcr

● High sensitivity
➤ Template: human gDNA: 3000 to 3 copies
➤ Primer (methyltransferase): FW #1458, RV #1459

pcr

● Amplification of long targets up to 5kb from Lambda DNA
➤ Template : Lambda DNA
➤ Primer: • FW #1469
• RV1kb #1472, 2kb #1473, 3kb #1474, 4kb #1499, 5kb #1498

pcr

Features
● Storage and Heat Endurance: 21 days in 37°C stable.
● Super high speed : Complete 2 kb in 60 seconds ( 30kb ~ 50 kb/sec in extension ).
● High sensitivity: Low copy number of template amplification.
● Amplification of long targets up to 5kb from Lambda DNA.
● Best balance between performance and value.

Cat Number : BCHF0500
Description

Hi Fi polymerase synthesizes higher yields of PCR product from genomic DNA, cDNA, bacterial cultures.It has 2.5 hours half life at 96oC and is easy to amplify PCR product of G-C rich or secondary structure DNA.2X Hi Fi Mix is optimized mixture. It contains Hi Fi polymerase, reaction buffer, dNTP and enhancer as 2-fold concentration. 2x Hi Fi mix is designed to allow the user to quickly and easily prepare the mixture of reaction. The 2x Hi Fi mix can amplify PCR products up to 10-15 kb and the products can be cloned into T-vector directly.

2X Hi Fi Mix
containing 3.6mM MgCl2
Application
Target of DNA fragments is up to 20 kb Proof reading function Amplification of genomic DNA

pcr

Cat Number: BCHT0500
Description

Hot start Taq DNA Polymerase is designed for Real-Time PCR and Hot- start PCR. It is modified with a special inhibition of PCR at room temperature. This will p r e ve nt p ri m e r d i me r s a n d o t he r a rt i fac ts . 2X Hot Start mix is optimized mixture. It contains Hot Start Taq polymerase, reaction buffer, dNTP and enhancer as 2-fold concentration. 2x Hot Start mix is designed to allow the user to quickly and easily prepare the mixture of reaction. The 2x Hot Start mix can amplify PCR products up to 5 kb and the products can be cloned into T-vector directly.

2X Hot Start mix
Containing 3.6 mM MgCl2
Application
Target of DNA fragments is up to 3-5 kb.
Hot Start reaction.
Real Time PCR.

Cat Number: BCP0500/ BCR0500
Description

2x PCR mix is optimized mixture. It contains Taq polymerase, reaction buffer, dNTP and enhancer as 2-fold concentration. 2x PCR mix is designed to allow the user to quickly and easily prepare the mixture of reaction. The PCR mix may amplify products up to 3 kb and the products can be cloned into T-vector directly. 2X Redy Mix is optimized mixture. It contains Taq polymerase, reaction buffer, dNTP, enhancer and red dye as 2-fold concentration. 2x Redy mix is designed to allow the user to quickly and easily prepare the mixture of reaction and ready loading.

Functional Assay
It is tested for performance in the polymerase chain reaction (PCR) to amplify a 500 bp region of the actin cDNA gene. The resulting PCR product is visualized on an ethidium bromide-stained agarose gel.



Applications
• Amplification of DNA fragments up to 5 kb.
• Labelling of PCR products with modified nucleotides (biotin-dUTP, fluorescein-dUTP).
• Cycle sequencing.
• Generation of PCR product for TA cloning.

pcr

Cat Number: BCPF0500
Description

Pfu DNA polymerase is a thermostable enzyme isolated from Pyrococcus furiousus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5'-3' direction. Pfu DNA polymerase possesses 3'-5' exonuclease (proofreading) activity. Base misincorporation is rapidly excised by the proofreading activity of the polymerase. Pfu DNA polymerase is recommended for PCR and primer extension reactions that require high-fidelity. The fragments of Pfu DNA polymerase generated are blunt-ended.
2X Pfu mix is optimized mixture. It contains Pfu polymerase, reaction buffer, dNTP and enhancer as 2-fold concentration. 2x Pfu mix is designed to allow the user for quickly and easily preparing reaction mixture. The 2x Pfu mix can amplify PCR products up to 3-5 kb.

2X Pfu mix
containing 50mM MgCl2



Applications
Amplification of DNA fragments is up to 7.5 kb Proof reading function.

pcr

Cat No Description qPCR Instruments
BCQPCR1-R 2X qPCR master mix with ROX ABI,7000,7300,7700 7900,stepOne Plus
StepOne™

Eppendorf Realplex 4
ABI7500
Stratagene Mx3000,
Mx3005, Mx4000
pcrmix
BCQPCR-IC 2x qPCR master mix BioRad CFX96 Roche LightCycler 480
MJ Research Opticon
and Opticon 2
MJ Research Chromo
4 Corbett Rotor-gene
600,3000
Eppendorf Realplex
2Product Application

pcrmix


Description

2x qPCR Master mix is designed for quantitative real-time analysis of DNA samples. • Strong signals and high sensitivity due to fluorescent dye.
High specificity - no primer dimers, no NTC signal.
Optimized 2x qPCR Master mixes for different real-time PCR instruments. Master mix formulations are optimized for different machines.

2x qPCR Master mix is ideally suited for:
Gene expression analysis.
Microarray validation.
Viral load determination qRT-PCR.

Components

2x qPCR Master mix is a 2X mixture of dNTPs, Hotstart Taq polymerase, MgCl2, fluorescent detection dye, reference dye (optional), and buffer components.

RT PCR KITS


Cat Number : BC9k-005-0005
Description

High RT Kit includes a recombinant genetically engineered version of a thermostable M-MULV reserve transcriptase Since this is more thermostable than M-MLV, the synthesis cDNA is possible over 50ºC and this is modified enzyme shows an optimal activity at 50ºC. In addition, the synthesis of cDNA is achieved efficiently because 2nd structure of RNA can be retarded more easily comparing to that in low temperature.

Cat Number : BR1041
Description

M-MLV Reverse Transcriptase (RT) is a genetically modified M-MLV RT. It differs from the M-MLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a ribonuclease H activity specific to RNA in RNA-DNA hybrids. M-MLV Reverse Transcriptase has significantly lower RNase H activity than Avian Myeloblastosis Virus (AMV) reverse transcriptase. Store Buffer20m M Tris-HCl (pH 7.5), 200 mM NaCl,0.1 mM EDTA,1 mM DTT, 0.01% NP-40, 50% glycerol.

Cat Number : BCGB100
Description

M-MLV cDNA Synthesis kit provides a sensitive and easy-to-use solution for two-step RT-PCR. This kit includes five tubes comprehensive of the reagents required for successful RT-PCR. The M-MLV H-reverse transcriptase is optimized for reliable cDNA synthesis over a wide dynamic range of input RNA. The enzyme is exceptionally well with a wide variety of targets.

Application:
1.First-strand cDNA synthesis for subsequent PCR or real-time PCR.
2.RT-PCR validation of gene expression data obtained from microarray experiments.
3.RT-PCR validation and quantification of gene silencing by RNA interference.

Kit contents
M-MLV RTase H-
5x RT buffer (contain dNTP mix)
5x RNA protector
oligodT (15mer)
Random primer (6mer)

Two step RT-PCR result:
kit

Cat Number : BCRT30100
Description

Super RT III Reverse Transcriptase, is an RNA-dependent DNA polymerase and with reduced RNase H activity and increase thermal stability. The Super RT III Reverse Transcriptase can synthesize 9.5kb products and provide high specificity ,high yields and more fulllength cDNA.
Unit description
One unit of activity is the amount of enzyme required to incorporate 1 nmole of dNTP into an acid-insoluble form in 10 minutes at 37°C using polyA-oligo (dT) as template and primer.

Supplied 5x RT buffer
TrisHCl, pH 8.3
KCl
MgCl 2
DTT

Storage buffer
50 mM TrisHCl, pH 8.3
100 mM NaCl
1 mM EDTA
0.1 mM DTT
0.1% Triton X-100
50% glycerol

Activity Assay 200 units of enzyme are used to produce cDNA from 1µg of a 1.2kb control RNA. The minimum specification is 120ng of first strand cDNA made from 1µg of RNA. The cDNA product must be >90% full length.

Cat Number : BC2RT0500
Description

2X RT master mix is designed for convenient and efficient cDNA synthesis. The 2X pre-mixed reagent containing M-MLV RTase, Random 6mers, oligo dT ,dNTP mixture and reaction buffer. The reverse transcription reaction just add RNA and water. The protocol is easy and the reaction can be completed in short time.

2X RT Master Mix
Containing M-MLV reverse transcriptase, reaction buffer, dNTP, oligo dT primers, random hexamers and stabilizer.

Application
cDNA synthesis
PCR screening
Real-time PCR

kit2

Lane 1-3 is soybean gene βactine, Lane 4-6 is Ecoli gene
1: M-MLV RTase + oligo dT
2: 2X master mix
3: M-MLV RTase + specific gene primer M:100 bp DNA ladder
4: M-MLV RTase + random 6mer
5: 2X master mix
6: M-MLV RTase + specific gene primer

AGAROSE LE


agarose

Cat Number : BC0015
Description

All applications for agarose take advantage of the specialcharacteristics of the marcoreticular gel. It is used as a sieve or support through whichbiological macromolecules such as proteins or nucleic acid can pass. Larger particles,such as viruses and subcellular fragments,are also able to move through the gelnetwork.Agarose LE is optimized for pulsedfieldelectrophoresis. This product has anexceptionally low EEO and high gel strength,both of which facilitate the preparation of low concentration gels for resolving Largefragments (>20kb). Gel strength (1.5%)>2200g/cm2.

GOOD VIEW DYE


Cat Number : HGV-II
Discription

GoodView TM is a new nucleic acid stain, an alternative to the traditional ethidium bromide (EB) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 268 nm and another at 294 nm. In addition, it has one visible excitation at 491 nm. The Fluorescence emission of GoodView TM bound to DNA is centered at 530 nm.

Protocol
1. Prepare 100 ml of agarose gel solution (concentration from 0.8~3%) in a 250 ml flask and mix it thoroughly. Place the flask in the microwave, heat it until the solution is completely clear and no small floating particles are visible (about 2~3 minutes).
2. Add 2-5ul of GoodViewTM to the gel solution. Swirl the flask gently to mix the solution and avoid forming bubbles.
3. While the gel solution cools, pour it into the gel tray until the comb teeth are immersed about 1/4~1/2 into the gel solution.
4. Allow the agarose gel to cool until solidified. Load samples on the gel and perform electrophoresis.
5. Detect the bands under UV illumination.

Store at 4℃ for 2 years
Comparative sensitivity test of GV and EB
1. Sensitivity test of GoodViewTM Nucleic Acid Stain Template:5~70 ngλDNA(λDNA diluted to different concentrations) GoodView:add 5 μlGoodView to 100 ml 1% agarose gel solution.
2. Sensitivity test of EB Template:5~70 ngλDNA(λDNA diluted to different concentrations) EB:add 5 μl EB (10 mg/ml) to 100 ml 1% agarose gel solution, to make the final concentration to 0.5 ng/μl.

good1

The result of electrophoresis demonstrates GV is almost as sensitive as EB.



IPTG


Cat Number : BC0168
Discription

iptg

TRIZOL

tri

Cat Number : BR100
Description

The TRAzol RNA Purification Kit provides a simple, reliable, and rapid method for isolating high–quality total RNA from a wide variety of samples, including animal and plant cells and tissue, bacteria, and yeast. The kit utilizes the strong lysis capability of TRAzol Reagent. TRAzol Reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components. Addition of chloroform followed by centrifugation, separates the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After transfer of the aqueous phase, the RNA is recovered by precipitation with isopropyl alcohol. After removal of the aqueous phase, the DNA and proteins in the sample can be recovered by sequential precipitation. Precipitation with ethanol yields DNA from the interphase, and an additional precipitation with isopropyl alcohol yields proteins from the organic phase. Copurification of the DNA may be useful for normalizing RNA yields from sample to sample. Total RNA isolated by TRAzol Reagent is free of protein and DNA contamination. It can be used for Northern blot analysis, dot blot hybridization, poly (A)+ selection, in vitro translation, RNase protection assay, and molecular cloning.

Features
The most classic formula
The most widely used
The most stable yield

Storage
Store at 2-8°C, protect from light for up to 12 months.

RNase


rnase

Cat Number : BC0473A
Description

A major application for Ribonuclease A (RNase A) is the removal of RNA from preparations of plasmid DNA. In this application, the presence of DNase activity as an impurity is a concern. The boiling-water bath method used to eliminate contaminating DNase activity has proven unreliable. For this reason, developed a proprietary chromatographic preparation method for elimination of DNase activity.

RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. RNase A can be inhibited by alkylation of His12 or His119 , which are present in the active site of the enzyme. Activators of RNase.

Molecular mass: 13.7 kDa (amino acid sequence)
Extinction coefficient: E1% = 7.1 (280 nm)
Isoelectric point: pI = 9.6
Optimal temperature: 60 °C (activity range of 15–70 °C)
Optimal pH: 7.6 (activity range of 6–10)
Inhibitors: ribonuclease inhibitor
The chromatographically purified product is supplied as an essentially salt-free lyophilized powder.
Activity (Kunitz): ≥60 units/mg protein.
Storage/Stability
This product remains active for at least 3 years when stored properly at –20 °C. RNase A is a very stable enzyme and solutions have been reported to withstand temperatures up to 100 °C. At 100 °C, an RNase A solution is most stable between pH 2.0 and 4.5.

Procedure
A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 0.2 mg/ml.

Cat Number : BC0474
Description

rnase

rnase

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